According to details for the upcoming My Brother, My Brother and Me live show tour, the My Brother, My Brother and Me show–launched on the now-defunct Seeso and now hosted on VRV–has been renewed for a second season:
Justin, Travis, and Griffin McElroy are the force behind a series of very successful podcasts, most notably MY BROTHER MY BROTHER AND ME and THE ADVENTURE ZONE. Each get upwards of 300k unique downloads per episode, and regularly chart on iTunes. MY BROTHER MY BROTHER AND ME has also launched as a Seeso series, and will be renewed for a second season (or more) shortly. Their tour dates sell out immediately, and regularly crash the servers of their ticketing partners.
While there has been discussion on Twitter about a potential second season, no official announcement has been released. However, this description posted March 13, 2018 seems to suggest confirmation. I have reached out to the podcast for any additional information they might be able to provide.
UPDATE 5/17/18: VRV has commented on the second season via tweet:
Originally posted 3/13/18